The Invisible Living World: Beyond Our Naked Eye
Introduction
- Our eyes can only see objects above a certain size. Tiny living things, too small to see, remained hidden until special tools were invented.
- A lens (a curved piece of glass, thick in the middle and thin at the edges) was used to make small things look bigger.
- Over time, lenses improved, leading to the invention of microscopes, which revealed a hidden world of tiny creatures called organisms.
The Discovery of the Microscopic World
Magnifying Glass: A simple tool that makes small objects look bigger. For example, looking at an ant through a magnifying glass shows its body details clearly.
Microscope: A powerful tool that magnifies objects 200-300 times or more, allowing us to see tiny organisms.
Robert Hooke (1655):
- Published a book called Micrographia.
- Used a microscope to observe a thin slice of cork and saw tiny, empty spaces that looked like a honeycomb.
- Named these spaces cells, the first time this term was used in science.
Antonie van Leeuwenhoek:
- Made better lenses and microscopes.
- First to see and describe tiny living things like bacteria and blood cells.
- Known as the Father of Microbiology.
What is a Cell?
- Cells are the basic building blocks of all living organisms (plants, animals, and microorganisms).
- All organisms, whether tiny or large, are made of cells.
- Cells can only be seen under a microscope because they are very small.
Structure of a Cell
1. Main Parts of a Cell:
Cell Membrane: A thin outer layer that surrounds the cell, separating it from other cells. It is porous, allowing essential materials to enter and waste to exit.
Cytoplasm: The jelly-like substance inside the cell where most life processes happen. It contains components like carbohydrates, proteins, fats, and minerals.
Nucleus: A small, round structure inside the cell, covered by a thin membrane. It controls all cell activities and regulates growth.
2.Plant Cells:
- Have an extra outer layer called the cell wall, which gives strength and rigidity.
- Contain plastids (e.g., chloroplasts with chlorophyll for photosynthesis, making them green).
- Have a large vacuole to store substances, remove waste, and maintain cell shape.
3.Animal Cells:
- Do not have a cell wall or chloroplasts.
- May have small vacuoles for storing substances.
4.Bacterial Cells:
- Have a cell wall but no well-defined nucleus. Instead, they have a nucleoid (a region where genetic material is stored).
Activity 2.2: Observing Onion Peel Cells
Steps:
1. Take an onion bulb, cut it, and peel off a thin, transparent layer (onion peel).
2.Place the peel in a petri dish with safranin (a red stain) for 30 seconds to color the cells.
3.Rinse the peel in water to remove extra stain.
4.Place the peel on a glass slide, add a drop of glycerin (to prevent drying), and cover with a coverslip.
5.Observe under a microscope.
Observation: Onion peel cells are rectangular, closely packed with no spaces, and have a cell wall, cell membrane, cytoplasm, and nucleus.
Activity 2.3: Observing Human Cheek Cells
Steps:
1. Rinse your mouth with water.
2.Gently scrape the inside of your cheek with a toothpick.
3.Place the scraped material in a drop of water on a slide and spread it.
4.Add a drop of methylene blue (a blue stain) to improve visibility.
5. Add glycerin, place a coverslip, and observe under a microscope.
Observation: Cheek cells are polygon-shaped, with a cell membrane, cytoplasm, and nucleus. Unlike plant cells, they lack a cell wall and chloroplasts.
Differences Between Plant and Animal Cells
Feature | Plant Cells | Animal Cells |
---|---|---|
Cell Wall | Present (provides rigidity) | Absent |
Chloroplasts | Present (for photosynthesis) | Absent |
Vacuole | Large, helps store substances | Small or absent |
Shape | Usually rectangular | Often irregular or polygonal |
Variation in Cell Shape and Structure
Cells have different shapes and structures based on their functions:
- Muscle Cells: Spindle-shaped, help in movement (e.g., pushing food in the digestive system).
- Nerve Cells (Neurons): Long and branched, carry messages across the body.
- Plant Cells: Can be rectangular, elongated, oval, or tube-like (e.g., tube-like cells carry water in plants).
The shape and structure of a cell are related to its specific function.
Levels of Organization in Living Organisms
- Cell: The basic unit of life (like a brick in a wall).
- Tissue: A group of similar cells performing a specific function.
- Organ: Different tissues working together (e.g., stomach).
- Organ System: Several organs working together (e.g., digestive system).
- Organism: All organ systems together form a complete living being (e.g., a human or plant).
- Example: In the digestive system, muscle cells in the food pipe push food, while stomach cells produce digestive juices to break it down.
Multicellular vs. Unicellular Organisms
1. Multicellular Organisms: Made of many cells (e.g., plants, animals, humans).
- Cells have specialized functions but work together for survival.
- Example: The ostrich egg yolk is the largest known single cell (130-170 mm in diameter).
2.Unicellular Organisms: Made of one cell (e.g., bacteria, Amoeba).
- The single cell performs all life functions.
Microorganisms
Microorganisms (or microbes) are tiny organisms invisible to the naked eye, seen only under a microscope (100-400 times magnification).
Types of microorganisms:
1.Bacteria: Unicellular, have a cell wall, no defined nucleus (nucleoid instead).
2.Protozoa: Unicellular, move and feed in water or soil.
3.Fungi: Unicellular (e.g., yeast) or multicellular (e.g., mould), have a cell wall but no chloroplasts.
4.Algae: Unicellular or multicellular, make food using sunlight (photosynthesis).
5.Viruses: Acellular (not made of cells), multiply only inside a host cell, can cause diseases.
Activity 2.4: Observing Pond Water
Collect pond or stagnant water, place a drop on a slide, cover with a coverslip, and observe under a microscope.
Observation: Tiny moving organisms (e.g., protozoa, algae) are visible.
Activity 2.5: Observing Soil Suspension
Mix moist soil with water, let it settle, take a drop from the top layer, and observe under a microscope.
Observation: Small moving organisms like those in pond water, showing microbes live in soil too.
Role of Microorganisms in Our Lives
1. Cleaning the Environment
Activity 2.7: Mix fruit/vegetable peels with soil, cover, and observe after 2-3 weeks.
- Result: Peels turn into dark, nutrient-rich manure due to microbes (fungi, bacteria) breaking them down.
- Role: Microbes decompose plant and animal waste, recycling nutrients back to the soil, helping plants grow.
Example: Gardeners collect dry leaves in pits to make natural manure.
Ancient Indian texts (e.g., Vedas) mention tiny entities called Krimi (visible and invisible) and their beneficial/harmful effects.
2. Microbes as a Source of Biogas
- Some bacteria live in oxygen-free environments and decompose waste, producing biogas (a mix of methane and carbon dioxide).
- Biogas is used for cooking, heating, electricity, and running vehicles.
- Dr. Ananda Mohan Chakrabarty: Developed a bacterium in 1971 to break down oil spills, helping clean the environment.
3. Microorganisms in Food
Activity 2.8: Yeast in Dough:
- Mix flour, sugar, and yeast in bowl A; only flour in bowl B. Knead with warm water, cover, and observe after 4-5 hours.
- Result: Dough in bowl A rises, becomes fluffy, and smells different due to yeast (a fungus).
- Why: Yeast respires, breaking down sugar to release carbon dioxide (makes dough fluffy) and a little alcohol (gives smell).
- Uses: Yeast is used to make bread, cakes, and pastries. Bacteria like Lactobacillus help make idli, dosa, and bhatura.
Activity 2.9: Curd Formation:
- Add curd to lukewarm milk (bowl A) and cold milk (bowl B). Keep A warm, B cool, and observe after a few hours.
- Result: Milk in bowl A turns into sour curd; bowl B remains mostly unchanged.
- Why: Lactobacillus bacteria in curd feed on milk sugar (lactose), producing lactic acid, which curdles milk. They grow better in warm conditions.
Why Pickles Don’t Spoil: High salt or sugar in pickles/murabbas prevents microbe growth.
4. Microorganisms in Agriculture
Rhizobium Bacteria:
- Live in root nodules of legumes (e.g., beans, peas).
- Trap nitrogen from the air and convert it into a form plants can use, improving soil fertility.
- Farmers rotate legume crops to naturally enrich soil with nitrogen.
5. Microalgae: Tiny Helpers in Water
Microalgae: Tiny plant-like organisms in water, soil, or air that make food via photosynthesis.
Roles:
- Produce over half of Earth’s oxygen.
- Serve as food for aquatic animals.
- Used as health supplements (e.g., Spirulina, Chlorella) and in medicines.
- Help clean water and produce biofuel.
Spirulina Farming:
- Grow in a glass tank with pond water, away from direct sunlight.
- Stir twice a week, harvest after 3-6 weeks by filtering.
- Spirulina is a superfood with high protein and vitamin B12.
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