Chapter 2: Cell — The Building Block of Life
15 Short Answer Questions + 10 Long Answer Questions | Theory & Application Both
5 Marks LAQ
CBSE Pattern
Diagrams Covered
Experiment-Based
Osmosis & Diffusion
How to Use This Q&A Sheet
This Q&A set covers all important concepts from Chapter 2: Cell — The Building Block of Life as per the Class 9 Science syllabus. Every question has a complete, exam-ready answer.
Read each question, cover the answer, and try to write it on your own first. Compare with the given answer to fill gaps. For LAQs, learn the steps in order — examiners award marks step-by-step.
- [Theoretical] questions test your understanding of definitions, concepts, and explanations.
- [Practical/Application] questions are based on experiments, real-life situations, or “why does this happen?” reasoning.
- Key biology terms are in bold throughout. Learn these for full marks.
- Questions marked with ⭐ are very frequently asked in school exams.
Short Answer Questions — 15 Questions (2–3 Marks Each)
🌿 Section A — Cell Structure & Discovery (Q1–Q5)
Formula: Size of one cell = 4000 ÷ 20
∴ Size of one onion cell = 200 µm
The total magnification of the microscope = 10 × 10 = 100X. This means the cell appears 100 times larger than its actual size. A microscope is essential for observing such small structures.
🌿 Section B — Cell Membrane, Cell Wall & Osmosis (Q6–Q10)
- In plain water: Plain water is a hypotonic solution compared to the cell sap inside potato cells. Water moves into the cells by osmosis, making the potato swell.
- In salt solution: The concentrated salt solution is hypertonic compared to the cell sap. Water moves out of the cells by osmosis, making the potato shrink and become limp.
The cell membrane acts as the selectively permeable barrier that allows water to pass but not the salt or sugar molecules.
🌿 Section C — Cell Organelles & Cell Division (Q11–Q15)
| Feature | Prokaryotic Cell (आदिकोशिकीय) | Eukaryotic Cell (सुकोशिकीय) |
|---|---|---|
| Nucleus | No well-defined nucleus; DNA in nucleoid region | Well-defined nucleus with nuclear membrane |
| Membrane-bound organelles | Absent | Present (e.g., mitochondria, ER) |
| Cell size | Smaller (1–10 µm) | Larger (10–100 µm) |
| Example | Bacteria | Plant cell, Animal cell |
- Chloroplasts (हरितलवक) — contain the green pigment chlorophyll; responsible for photosynthesis (making food using sunlight).
- Chromoplasts (वर्णलवक) — contain yellow, orange, or red pigments; give bright colours to flowers and fruits to attract pollinators and seed dispersers.
- Leucoplasts (अवर्णलवक) — colourless plastids that store food materials such as starch, oils, or proteins (e.g., starch storage in potato cells).
Long Answer Questions — 10 Questions (5 Marks Each)
🌿 Section A — Cell Basics, Microscopy & Membrane (Q1–Q4)
- The membrane has a lipid bilayer — two layers of fat (lipid) molecules arranged with their water-attracting (hydrophilic) heads pointing outward and their water-repelling (hydrophobic) tails pointing inward toward each other.
- Various protein molecules are embedded throughout this lipid bilayer, scattered like tiles in a mosaic — hence the name “mosaic”.
- The lipid and protein molecules can move sideways, flip, and rotate within the layer — making the membrane fluid.
- The embedded proteins act as gatekeepers — they form channels or pumps that help specific substances (like glucose, ions, or large molecules) pass through the membrane in a controlled way.
- The membrane is selectively permeable — small, non-polar molecules like oxygen and carbon dioxide can pass freely; water passes through special protein channels called aquaporins; large or charged molecules can only enter with the help of transport proteins.
This selective control allows the cell to take in nutrients, remove waste, and maintain a stable internal environment (homeostasis).
- Materials needed: Two potato pieces of equal size, Beaker A with plain water, Beaker B with 20% salt solution, a weighing balance.
- Procedure: Measure and record the initial weight of both potato pieces. Place one piece in Beaker A (plain water) and the other in Beaker B (salt solution). Leave undisturbed for about one hour.
- Observation — Beaker A: The potato piece swells and its final weight is greater than the initial weight. Water has moved into the potato cells.
- Observation — Beaker B: The potato piece shrinks and its final weight is less than the initial weight. Water has moved out of the potato cells.
- Conclusion: The cell membrane is selectively permeable — it allows water to pass but not sugar or salt. Water moves by osmosis from a region of higher water concentration (dilute solution) to lower water concentration (concentrated solution). Plain water is hypotonic to the cell sap → water enters. Salt solution is hypertonic to the cell sap → water exits.
- Present in all living cells (plant, animal, fungi, bacteria)
- Made of lipids and proteins
- Selectively permeable — controls what enters and exits
- Thin (~7–10 nm) and flexible
- Regulates cell communication and transport
- Present only in plants, fungi, and bacteria — absent in animals
- Made of cellulose (in plants)
- Freely permeable — allows water and dissolved minerals to pass
- Thick and rigid — provides structural support
- Maintains cell shape; prevents bursting from excess water
Why a plant cell needs both:
- The cell wall gives rigidity and structural support — allowing plants to stand upright, resist wind, and maintain leaf/flower shape.
- The cell membrane (inside the cell wall) regulates the movement of specific molecules, controlling metabolism.
- Together, both membranes work to allow selective absorption of water and nutrients while maintaining the cell’s shape under varying environmental conditions.
- Isotonic solution (समपरासारी): The solute concentration of the external solution equals the solute concentration inside the cell. No net movement of water occurs. The cell remains unchanged in size.
- Hypotonic solution (अल्पपरासारी): The solute concentration of the external solution is less than the inside of the cell (i.e., the external solution is more dilute). Water moves into the cell by osmosis. An animal cell swells and may eventually burst (lyse) because it has no rigid cell wall to limit expansion.
- Hypertonic solution (अतिपरासारी): The solute concentration of the external solution is greater than the inside of the cell (i.e., the external solution is more concentrated). Water moves out of the cell by osmosis. An animal cell shrinks (crenation) as it loses water.
Isotonic → No change in cell size
Hypotonic → Cell swells (water enters)
Hypertonic → Cell shrinks (water exits)
∴ Concentration of surrounding solution determines cell size changes
Note: In plant cells placed in hypertonic solution, the cell does not shrink because the rigid cell wall maintains its outer shape — only the inner content (cytoplasm) shrinks away from the wall (plasmolysis).
🌿 Section B — Cell Organelles in Detail (Q5–Q7)
- Structure: Each mitochondrion has two membranes — an outer smooth membrane and an inner membrane that is deeply folded into finger-like projections called cristae.
- The cristae increase the surface area available for chemical reactions, which greatly improves the efficiency of energy production.
- The space enclosed by the inner membrane is filled with a semi-fluid matrix containing enzymes, its own DNA, and its own ribosomes.
- Function — Cellular Respiration: In mitochondria, glucose and other food molecules are broken down using oxygen in a process called cellular respiration. The energy released is stored in the form of ATP (Adenosine Triphosphate) molecules.
- ATP acts as the energy currency of the cell — it is used to power all cellular activities including muscle contraction, protein synthesis, and active transport.
- (a) Endoplasmic Reticulum (ER) — अन्तर्द्रव्यी जालिका: The ER is a large network of membranes that spreads throughout the cytoplasm and is connected to the outer nuclear membrane. It has two types:
- Rough ER (RER): Has ribosomes on its surface (looks rough); involved in protein synthesis and transport. Example: pancreatic cells that make digestive enzymes.
- Smooth ER (SER): Lacks ribosomes (looks smooth); involved in synthesis of lipids and hormones.
- (b) Golgi Apparatus — गॉल्जी उपकरण: Discovered by Camillo Golgi in 1898, this organelle consists of stacks of flattened, sac-like structures. It is functionally linked to the ER. Its key functions are:
- Modifies, sorts, and packages proteins and lipids received from the ER.
- Sends them to their destination — either outside the cell (secretion), to the cell membrane, or to form lysosomes.
- Called the cell’s “post office” because it addresses and dispatches cellular materials.
- (c) Lysosomes — लाइसोसोम: These are small, single membrane-bound sacs filled with powerful digestive enzymes. They serve as the cell’s “clean-up system”:
- Break down old, worn-out organelles and cellular waste materials.
- Digest foreign particles (bacteria) that enter the cell.
- The breakdown products are recycled back into the cytoplasm for reuse.
| Feature | Mitochondria | Chloroplasts |
|---|---|---|
| Location | All eukaryotic cells (plant + animal) | Only in plant cells (and some algae) |
| Membranes | Double membrane; inner membrane forms cristae | Double membrane; inner structures form grana (stacks) |
| Internal fluid | Matrix (contains enzymes, DNA, ribosomes) | Stroma (contains enzymes, DNA, ribosomes) |
| Own DNA | Yes — circular DNA | Yes — circular DNA |
| Own ribosomes | Yes | Yes |
| Main function | Cellular respiration → produces ATP (energy) | Photosynthesis → produces glucose (food) using sunlight |
| Process | Breaks down food; uses oxygen | Makes food; releases oxygen |
Structural Similarities: Both are double membrane-bound organelles. Both contain their own circular DNA and ribosomes, meaning they can partially make their own proteins. These features suggest that both organelles evolved from ancient free-living bacteria through a process called endosymbiosis.
🌿 Section C — Cell Division & Cell Theory (Q8–Q10)
- Matthias Schleiden (1838) — a German botanist — proposed that all plants are made up of cells.
- Theodor Schwann (1839) — a German zoologist — extended this to animals, proposing that all animals are also made up of cells.
- Rudolf Virchow (1855) — a German scientist — added the third and most important principle: “Omnis cellula e cellula” — all new cells arise only from pre-existing cells.
- Together, these contributions form the Classical Cell Theory, which states:
- All living organisms are made up of one or more cells.
- The cell is the basic structural and functional unit of all living beings.
- All cells arise from pre-existing cells (through cell division).
- Unifying power of Cell Theory: It applies to every living organism — from a tiny bacterium (1 cell) to a giant blue whale (trillions of cells). It explains how life continues from generation to generation through cell division. It ties together biology, medicine, evolution, and genetics under one common principle.
| Feature | Mitosis (समसूत्री) | Meiosis (अर्धसूत्री) |
|---|---|---|
| Where it occurs | All body (somatic) cells | Only reproductive organs (testes/ovaries in animals; anthers/ovaries in plants) |
| Number of divisions | One division | Two consecutive divisions |
| Daughter cells produced | 2 daughter cells | 4 daughter cells |
| Chromosome number | Same as parent cell (diploid → diploid) | Half the parent’s chromosome number (diploid → haploid) |
| Genetic identity | Genetically identical to parent | Genetically diverse (creates variation) |
| Purpose | Growth, repair, maintenance | Produces gametes (sperm and egg) for sexual reproduction |
Importance of meiosis in sexual reproduction:
- Meiosis produces gametes with half the chromosome number. When two gametes (sperm + egg) fuse during fertilisation, the original chromosome number is restored in the offspring.
- Meiosis creates genetic diversity — children resemble their parents but are not identical to them, because different combinations of chromosomes end up in different gametes.
- Without meiosis, chromosome numbers would double with every generation, which would be fatal.
- When a large amount of sugar is added to the amla, the surrounding solution becomes very concentrated (hypertonic) — it has a very high solute (sugar) concentration and very low water concentration.
- If bacteria land on the murabba, the high sugar concentration outside the bacterial cells is greater than the concentration inside the bacterial cells.
- By the process of osmosis, water moves from the region of higher water concentration (inside the bacterial cell) to the region of lower water concentration (outside, i.e., the sugar solution).
- The bacteria rapidly lose water from their cells. Their cell contents shrink — this is called plasmolysis in plant/bacterial cells. The bacteria are essentially dehydrated.
- Without water, the bacteria cannot carry out metabolic activities and die or become inactive — they cannot cause spoilage. This is why the murabba stays preserved for a long time.
∴ Osmosis is the scientific basis of food preservation using salt and sugar
Key Terms & Quick Reference
| Key Term | Meaning |
|---|---|
| Osmosis | Diffusion of water across a selectively permeable membrane |
| Diffusion | Movement of particles from higher concentration to lower concentration (no membrane needed) |
| Prokaryotic cell | Cell with no well-defined nucleus or membrane-bound organelles (e.g., bacteria) |
| Eukaryotic cell | Cell with a well-defined nucleus and membrane-bound organelles (e.g., plant, animal) |
| Selectively permeable | Allows only certain substances to pass through |
| ATP | Adenosine Triphosphate — the energy currency of the cell, produced in mitochondria |
| Chlorophyll | Green pigment in chloroplasts; absorbs sunlight for photosynthesis |
| Plasmolysis | Shrinkage of cell contents away from cell wall when placed in hypertonic solution |
| Chromatin | Thread-like mass of DNA and proteins in the nucleus; condenses into chromosomes during cell division |
| Nucleoid | Region in prokaryotic cells where DNA is located (no nuclear membrane) |
| Contact inhibition | Normal cells stop dividing when they touch neighbouring cells |
| Cell sap | Watery fluid stored inside the vacuole of plant cells |
Common Exam Mistakes to Avoid
For SAQs (2–3 marks): Write 3–4 sentences with key terms in bold. Always define the term first, then explain. For LAQs (5 marks): Use numbered points or a table format. Include all sub-parts — examiners check each point separately. Always write the conclusion or significance of the concept at the end. For diagram-based questions: Label at least 4–5 parts, draw neatly with a pencil, and give a proper title. Practice writing answers in the exam time limit — quality matters more than length.

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